| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Methanobrevibacter oralis | GATGGCAAAACAAACTGCTGT | AGGCCTTGTTCTTGCTCCAT | 58.17 | 59.59 | 181 | 138 |
100.00%
|
100.00%
|
| Primer 2 | Methanobrevibacter oralis | GATGGCAAAACAAACTGCTGT | GGCCTTGTTCTTGCTCCATC | 58.17 | 59.19 | 180 | 138 |
100.00%
|
100.00%
|
| Primer 3 | Methanobrevibacter oralis | GATGGCAAAACAAACTGCTGT | GGCCTTGTTCTTGCTCCAT | 58.17 | 58.03 | 180 | 138 |
100.00%
|
100.00%
|
| Primer 4 | Methanobrevibacter oralis | GATGGCAAAACAAACTGCTGT | ACCAAAAGGCCTTGTTCTTGC | 58.17 | 59.86 | 187 | 138 |
100.00%
|
100.00%
|
| Primer 5 | Methanobrevibacter oralis | GATGGCAAAACAAACTGCTGT | AAGGCCTTGTTCTTGCTCCAT | 58.17 | 60.20 | 182 | 138 |
100.00%
|
100.00%
|
| Primer 6 | Methanobrevibacter oralis | GATGGCAAAACAAACTGCTGT | AAAGGCCTTGTTCTTGCTCC | 58.17 | 58.67 | 183 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Methanobrevibacter oralis | GCTGTTCAAAGGGAATTGTGC | AGACACCCCTAGACATTGCAG | 58.59 | 59.44 | 238 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Methanobrevibacter oralis | GGGACGTGGAAGTCAGAAGG | GCATTGTGCCCTTCTTCACC | 60.04 | 59.76 | 231 | 132 |
100.00%
|
100.00%
|
| Primer 9 | Methanobrevibacter oralis | GAGAACTGCACGTCGAACC | TTCCTCGCCAGCGTTTACC | 58.86 | 60.38 | 229 | 132 |
100.00%
|
100.00%
|
| Primer 10 | Methanobrevibacter oralis | TGCAGGCGTTGTGAAATCAG | GCAGCGCATGAAGTTACTGG | 59.41 | 59.90 | 243 | 131 |
100.00%
|
100.00%
|